The Genomics Core Laboratory in the Department of Microbiology, Immunology and Pathology is established to house both basic and translational genomics research, allow individual investigators access to state-of-the-art next-generation sequencing (NGS) technologies, conduct genomics experiments and bioinformatics analyses, and provide opportunities for education and training in genomics and bioinformatics.
Instruments
The Genomics Core Laboratory instrumentation includes:
- Qubit 2.0 Fluorometer for flurometric quantification of DNA, RNA and protein
- Bioanalyzer 2100 for quantification, size and purity assessment of DNA, RNA and protein
- TapeStation 4200 for quantification, size and purity assessment of DNA, RNA and protein
- BluePippin for DNA size selection/collection ranged from 100 bp to 50 kb with pulse-field
- Nextseq 2000 for single- or paired-end short-read ranged from 50 bp to 300 bp
- Covaris M550 (for shearing RNA/DNA)
Services
The Genomics Core Laboratory provides and supports a variety of NGS services and their following bioinformatics data analyses. Consultation before services is freely available and is strongly recommended.
Sample prep services include:
- Single cell transcriptomics
- 10x Chromium
- Parse
- mRNAseq
- Small RNA (tRNA, miRNA, snoRNA etc.)
- WGS (whole genome sequencing)
- DNA-methylation
- Metagenomics
- Microbiome
- Special projects (atypical samples, poor starting material etc. )
NGS services include:
- Sequencing on Nextseq 2000, Miseq
- Data Analysis
- Development of specialized tools
Visit, Consults and Service Requests
Genomics Core Laboratory is located at Basic Sciences Building Room 409 at Pathology Department. Investigators are welcome to visit, consult and request services of genomics and bioinformatics.
Contact
Ravi Sachidanandam, Ph.D.
Director, Genomics Core Lab
Dept. of Pathology, Microbiology and Immunology
Basic Sciences Building Room 408
Phone: (516) 527-4452
Email: rsachidanandam@nymc.edu
Lab Leadership
Humayun Islam, M.D., Ph.D.
Chairman of Pathology, Microbiology and Immunology
Basic Sciences Building Room 413
Phone: (914) 594-4150
Fax: (914) 594-4163
Email: humayun.islam@wmchealth.org
