Research

Dr. Huang's working field is focused on the specific regulation of cardiovascular function via nitric oxide (NO)-dependent and independent mechanisms, as a function of sex/estrogen. In particular, the signaling cascades responsible for the estrogen-specific regulation by a cytochrome P450-dependent mechanism are specifically assessed, in which epoxyeicosatrienoic acids (EETs) serve as estrogen targets to divergently initiate cardiovascular actions in both systemic and pulmonary circulations, as a function of pathophysiological status. Experiments are mainly conducted on microvessels (~100-200 µm) isolated from skeletal muscle, mesentery and coronary vascular beds of rats and mice. The functional changes involving vasodilator and constrictor responses are assessed. Also, experiments aimed to clarify possible mechanisms are performed by using molecular (PT-PCT, Western) and biochemical (HPLC, GC-MS) analyses, and transfection methods (si RNA) in the isolated vessels. Additionally, in vivo dynamically monitoring pulmonary artery pressure in rodents, associated with entire echocardiography analyses were newly established.

Publications

• Ishiko S, Koller A, Deng W, et. al. "Liposomal nanocarriers of preassembled glycocalyx restore normal venular permeability and shear stress sensitivity in sepsis: assessed quantitatively with a novel microchamber system." American journal of physiology. Heart and circulatory physiology, 327(2), (2024) H390-H398. doi: 10.1152/ajpheart.00138.2024
• Ishiko S, Huang A, Sun D, et. al. "Long-term efficacy of liposomal nanocarriers of preassembled glycocalyx in restoring cerebral endothelial glycocalyx in sepsis." Microvascular research, 154(), (2024) 104684. doi: 10.1016/j.mvr.2024.104684
• Ishiko S, Ben Rahoma G, Kandhi S, et. al. "Liposomal nanocarriers of preassembled glycocalyx expeditiously restore endothelial glycocalyx in endotoxemia." American journal of physiology. Heart and circulatory physiology, 325(4), (2023) H645-H655. doi: 10.1152/ajpheart.00196.2023
• Huang A, Kandhi S, Sun D, et. al. "Roles of Genetic Predisposition in the Sex Bias of Pulmonary Pathophysiology, as a Function of Estrogens : Sex Matters in the Prevalence of Lung Diseases." Advances in experimental medicine and biology, 1303(), (2021) 107-127. doi: 10.1007/978-3-030-63046-1_7
• Froogh G, Kandhi S, Duvvi R, et. al. "The contribution of chymase-dependent formation of ANG II to cardiac dysfunction in metabolic syndrome of young rats: roles of fructose and EETs." American journal of physiology. Heart and circulatory physiology, 318(4), (2020) H985-H993. doi: 10.1152/ajpheart.00633.2019
• Kandhi S, Alruwaili N, Wolin MS, et. al. "Reciprocal actions of constrictor prostanoids and superoxide in chronic hypoxia-induced pulmonary hypertension: roles of EETs." Pulmonary circulation, 9(4), (2019) 2045894019895947. doi: 10.1177/2045894019895947
• Huang A, Sun D. "Sexually Dimorphic Regulation of EET Synthesis and Metabolism: Roles of Estrogen." Frontiers in pharmacology, 9(), (2018) 1222. doi: 10.3389/fphar.2018.01222

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Teaching Responsibilities

• School of Medicine: Mammalian Physiology (Pregnant Physiology, Neonatal Physiology, and Gastrointestinal Physiology)
• Graduate School of Biomedical Sciences: Mammalian Physiology (Pregnant Physiology and Gastrointestinal Physiology)
• Touro College of Dental Medicine: Mammalian Physiology (Reproductive System, Pregnancy Physiology, and Gastrointestinal Physiology.